The Highly Embryogenic Brassica napus DH4079 Line Is Recalcitrant to Agrobacterium-Mediated Genetic Transformation

Brassica napus is a species of high agronomic interest, used as model to study different processes, including microspore embryogenesis. The DH4079 and DH12075 lines show low embryogenic response, respectively, which makes them ideal the basic mechanisms controlling embryogenesis induction. Therefore, availability protocols for genetic transformation these two backgrounds would help generate tools better understand this process. There are some reports in literature showing stable DH12075. However, no equivalent studies have been reported date. We explored ability plants be genetically transformed. As reference compare with, we same transform three previously successful B. with Agrobacterium tumefaciens analyzed response plants. Whereas responded transformation, were completely recalcitrant, not producing any single regenerant out 1784 explants transformed cultured. Additionally, an rhizogenes transient assay was performed on both lines, only DH12075, but seedlings, A. infection. propose that line recalcitrant Agrobacterium-mediated transformation.